Journal: Journal of nanobiotechnology

Article Title: Exosome-derived circUPF2 enhances resistance to targeted therapy by redeploying ferroptosis sensitivity in hepatocellular carcinoma.

doi: 10.1186/s12951-024-02582-6

Figure Lengend Snippet: Fig. 1 Exosomes derived from sorafenib-resistant Huh-7 cells induce an enhanced resistance to sorafenib in HCC cells. (A) Sorafenib-resistant cell line, Huh-7-SR, was established and confirmed by the IC50 value. (B) Co-culture systems were established to investigate the effects of sorafenib-resistant HCC cells on regular HCC cells. The group with additional GW4869 served as a negative control. (C) Cell viabilities of HCC cells co-cultured with Huh-7 or Huh- 7-SR cells under sorafenib stress were evaluated via CCK-8 assays. (D) Assessment of cell proliferation using colony formation assay in Huh-7 and Hep3B cells with the indicated co-culture model. (E) Concentration and size of exosome were measured by NTA. Exosomes were isolated from the supernatant of the culture medium of Huh-7-SR (Exo-SR) and Huh-7 (Exo-Norm) cells by ultracentrifugation. (F) The iconic cup-shaped double-layer closed vesicle structure of isolated exosome was confirmed by TEM. (G) Western blotting analysis of exosomal markers, including CD9, CD63, CD81, LAMP2, TSG101 and Calnexin. An exosome standard from HEK293 cell line was analyzed as a positive control, while Huh-7-SR cell lysate was performed as a negative control. (H) The fluorescent signal of PKH67 labeled Exo-SR was captured in Huh-7 and Hep3B cells. (I) CCK-8 assays were performed to assess the cell viability of Huh-7 and Hep3B cells treated with sorafenib (6 µM) while co-cultured with the indicated exosomes. (J) Colony formation assays were performed to assess the cell proliferation of Huh-7 and Hep3B cells. The data are presented as the mean ± SD of at least three independent experiments. ***P < 0.001

Article Snippet: A positive control was conducted using a standard sample of exosomes derived from HEK293 cells (Novus, USA), while a negative control was performed using Huh-7-SR cell lysate.

Techniques: Derivative Assay, Co-Culture Assay, Negative Control, Cell Culture, CCK-8 Assay, Colony Assay, Concentration Assay, Isolation, Western Blot, Positive Control, Labeling

Journal: Acta Neuropathologica

Article Title: Biomarkers for parkinsonian disorders in CNS-originating EVs: promise and challenges

doi: 10.1007/s00401-023-02557-1

Figure Lengend Snippet: Summary of EV-associated miRNAs in Parkinson’s disease

Article Snippet: Plasma , PD = 52 HC = 48 , PureExo Exosome Isolation (101Bio) , Exosomal RNA and Protein Extraction kit (101Bio) , qRT-PCR , miR-626, miR-505, miR-181c, miR-331-5p, miR-193-p, miR-196-p, miR-454, miR-125a-p, miR-137 , miR-331-5p , miR-505 , d AUC = 0.898 , [ ] .

Techniques: Isolation, RNA Extraction, TaqMan microRNA Assay, RNA Sequencing, Clinical Proteomics, Protein Extraction, Next-Generation Sequencing, Immunoprecipitation, Microarray, Hybridization

Journal: Molecular Biomedicine

Article Title: Progress in exosome associated tumor markers and their detection methods

doi: 10.1186/s43556-020-00002-3

Figure Lengend Snippet: Nucleic acid biomarkers in exosome for tumor diagnosis

Article Snippet: Copyright© 2015 Elsevier Inc. All rights reserved After purifying exosomes from plasma or cell culture supernatants via suitable isolated method, RNA can then be extracted by purification kits, such as SBI’s SeraMir kit, mirRCURY RNA Isolation Kit (Exiqon, Vedbaek, Denmark) [ ], Exosome Total RNA Extraction Kit (HansaBioMed), phenolisopropanol precipitation (Trizol, Invitrogen) or Exosome RNA Isolation Kit (Norgen Biotek).

Techniques: Mutagenesis

Journal: Molecular Biomedicine

Article Title: Progress in exosome associated tumor markers and their detection methods

doi: 10.1186/s43556-020-00002-3

Figure Lengend Snippet: Comparison of different exosome isolation methods

Article Snippet: Copyright© 2015 Elsevier Inc. All rights reserved After purifying exosomes from plasma or cell culture supernatants via suitable isolated method, RNA can then be extracted by purification kits, such as SBI’s SeraMir kit, mirRCURY RNA Isolation Kit (Exiqon, Vedbaek, Denmark) [ ], Exosome Total RNA Extraction Kit (HansaBioMed), phenolisopropanol precipitation (Trizol, Invitrogen) or Exosome RNA Isolation Kit (Norgen Biotek).

Techniques: Isolation, Gradient Centrifugation, Blocking Assay, Filtration, Chromatography, Marker, Binding Assay, Purification, Microarray, Chromatin Immunoprecipitation, In Situ, Concentration Assay

Journal: Molecular Biomedicine

Article Title: Progress in exosome associated tumor markers and their detection methods

doi: 10.1186/s43556-020-00002-3

Figure Lengend Snippet: The abundances and types of specific RNA classes present in exosome by NGS sequence . Copyright© 2015 Elsevier Inc. All rights reserved

Article Snippet: Copyright© 2015 Elsevier Inc. All rights reserved After purifying exosomes from plasma or cell culture supernatants via suitable isolated method, RNA can then be extracted by purification kits, such as SBI’s SeraMir kit, mirRCURY RNA Isolation Kit (Exiqon, Vedbaek, Denmark) [ ], Exosome Total RNA Extraction Kit (HansaBioMed), phenolisopropanol precipitation (Trizol, Invitrogen) or Exosome RNA Isolation Kit (Norgen Biotek).

Techniques: Sequencing